F1-X™Next-Generation Gibson Assembly
Same protocol. Validated fidelity. Better price.
Scientist-Led
Built by PhDs, Inventor-Advised
Made in USA
In-House Manufacturing & QC
NGS-Verified
Sequence-Validated Every Lot
Global Reach
7+ Partners Worldwide
PRICING
Simple, Transparent Pricing.
NEBuilder® HiFi: $18.60/rxn at 10-rxn scale — competitor pricing sourced from manufacturer website as of March 2026, subject to change.
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PERFORMANCE
Explore Performance Data
Proven Fidelity, Better Price
In internal benchmarks, achieves parity or higher fidelity than NEBuilder® HiFi, GeneArt™ Gibson Assembly HiFi, and the original Gibson Assembly® Cloning Kit in a 4-fragment head-to-head — at a fraction of the cost. Validated by Oxford Nanopore whole-plasmid sequencing.
Multi-Fragment Power
95–97% cloning efficiency maintained across 4, 7, 9, and 11 fragment assemblies in a single step. Demonstrated up to 14 fragments (9 kb lycopene pathway, de novo). No protocol changes.
Freeze-Thaw Robust
After 5 freeze-thaw cycles: ~95% efficiency, error rate 2.7–7.8 × 10⁻⁵ errors/bp — well below input synthetic DNA error rate. Glycerol formulation stays liquid at −20°C.
Automation Compatible
Single-tube, isothermal reaction drops directly into liquid-handling and LIMS-linked workflows. Consistent results in 96-well plates at miniaturized, sub-20 µL reaction volumes.
Developed with scientific input from Dr. Dan Gibson, inventor of Gibson Assembly® and Racer board member.
APP NOTE
Example: 14-Fragment Pathway Assembly
9 kb lycopene biosynthesis construct assembled in one reaction using F1-X™ — no vector isolation, no restriction digests, no gel purification.
THE RACER PLATFORM