COMING 2026
RC-X™ Rolling Circle Amplification
Microgram-scale DNA. No E. coli. No compromise.
>10 µg
DNA Yield
2–6 hr
Amplification Time
50 µg
Maximum Yield
Cell-Free
No E. coli Required
RC-X™ CAPABILITIES
Amplify what others can't.
IVT & mRNA Synthesis
Generate microgram-scale linearized template DNA for in vitro transcription without bacterial amplification. RC-X™ output is compatible with standard IVT kits and produces clean mRNA with reduced immunogenicity concerns from residual bacterial contaminants.
Antibody & Vaccine Discovery
Rapidly amplify variant libraries and candidate plasmids for transient transfection screening. RC-X™ eliminates the bottleneck of cloning and overnight culture, enabling same-day template preparation for high-throughput antibody discovery and vaccine construct evaluation.
Sequencing & Diagnostics
Produce high-purity template DNA for Oxford Nanopore or PacBio long-read sequencing directly from assembled constructs. RC-X™ delivers the quantity and quality needed for reliable full-length plasmid verification and diagnostic assay development.
APPLICATIONS
Where RC-X™ fits your workflow.
In Vitro Transcription & mRNA Synthesis
Generate rapid, high-yield DNA templates for IVT. By removing E. coli from the prep, RC-X™ lowers endotoxin risk, improves reproducibility, and compresses timelines from days to hours.
Antibody & Vaccine Discovery
Support high-throughput screening with isothermal, microgram-scale amplification — no colony picking or minipreps required, so design-to-test cycles run faster.
Sequencing & Diagnostics
Produce sequence-ready DNA with minimal background for Sanger or NGS. Low-contamination output aids assay development, variant detection, and construct validation.
RC-X™ Kit
RC-X™ uses proprietary rolling circle amplification (RCA) enzymology to produce microgram-scale quantities of plasmid DNA entirely in vitro. Skip bacterial transformation, overnight cultures, and miniprep columns. Simply add your assembled or sequence-verified template, incubate for 2–6 hours, and recover >10 µg of high-purity, low-endotoxin DNA ready for downstream applications including IVT, transfection, and sequencing.
- >10 µg DNA yield per reaction
- 2–6 hour amplification time
- Up to 50 µg maximum yield
- Cell-free — no E. coli required