F1-X™ Next-Gen Gibson Assembly
F1-X™ is our re-engineered one-pot DNA assembly kit designed for accuracy, speed, and performance. It delivers seamless multi-fragment builds in a high-fidelity, scarless format.
New protocols and guidance for speed, cost savings, and transparent troubleshooting.
Scarless joins; updated, high-fidelity enzyme system
Compatible with operons, fusions, libraries, and large constructs
Automation-friendly
100% money-back guarantee on Inventor's Lot purchase
Built for These Workflows
From pathway engineering to gene therapy vectors and pooled libraries, F1-X™ Next-Gen Gibson Assembly delivers reliable, one-step multi-fragment assembly with high-fidelity joins.
Strain Engineering
Build and iterate faster with fewer errors.
Gene Therapy Vectors
Precision cloning for viral vector development.
CRISPR Libraries
Seamless construction of gRNA and donor libraries.
Large DNA & Storage
Assemble and preserve high- integrity, multi-kilobase constructs.
Strain Engineering
Build and iterate faster with fewer errors.
F1-X™ Next-Gen Gibson Assembly enables rapid design–test cycles by assembling multi-gene operons, tunable promoters, regulatory cassettes, and pathway modules in a single, one-step reaction. It supports scarless joins across multi-fragment, so teams can iterate metabolic and synthetic circuits quickly—with fewer bottlenecks and higher fidelity.
Use Cases:
- Pathway prototyping in chassis organisms
- Cloning logic gates, feedback loops, or biosynthetic clusters
- Scarless assembly of gene + RBS + terminator units
Why It Works:
Fast, one-tube reactions reduce hands-on time, while high-fidelity enzymes reduce junction errors and preserve design logic.
Gene Therapy Vectors
Precision cloning for viral vector development.
F1-X™ Next-Gen Gibson Assembly is built for complex gene therapy constructs, including AAV and lentiviral expression cassettes. High-fidelity, scarless joins preserve regulatory elements, payloads, and UTRs across fragments—helping maintain ITR/LTR integrity and reducing design-related recombination risks.
Use Cases:
- Inserting payloads into AAV backbones
- Lentiviral constructs with promoters + payloads + marker + WPRE
- Assembling long polycistronic expression cassettes
Why It Works:
High-fidelity assembly with up to multiple fragments in one step allows you to customize vectors while keeping essential regulatory features intact.
CRISPR Libraries
Seamless construction of gRNA and donor libraries.
F1-X™ Next-Gen Gibson Assembly streamlines pooled or plate-based CRISPR assemblies. Assemble gRNA cassettes (U6 promoter, gRNA scaffold, tracrRNA) or HDR donors with homology arms and payloads in a single, one-step reaction. High-fidelity, scarless junctions help maintain guide architecture and minimize ligation artifacts multiple fragments.
Use Cases:
- Building pooled or arrayed gRNA libraries
- HDR donor templates (homology arms + payload)
- Multiplexed cloning of gRNA + Cas9 + marker
Why It Works:
Scar-free joins and reliable multi-fragment assembly reduce the risk of dropout, enabling higher library fidelity and consistent functional output.
Large DNA & Storage
Assemble and preserve high-integrity, multi-kilobase constructs.
For synthetic DNA storage, enzyme pathway archiving, and vector backbone refactoring, F1-X™ Next-Gen Gibson Assembly delivers high-fidelity multi-fragment builds with 3-5+ kb per fragment, scarless junctions, and low error rates—built for scenarios where structural accuracy and integrity are non-negotiable.
Use Cases:
- Joining 5–10+ kb fragments for DNA storage or archiving
- Assembly of stable plasmids encoding full-length multi-domain genes
Why It Works:
The high-fidelity polymerase and ligation-optimized mix preserve long reads and structure. No need for cleanup, gel extraction, or overlap re-design.
High Value Applications Summary
Antibody and Enzyme Discovery
Discovery teams screening large antibody or enzyme panels need fast, reliable cloning. F1-X™ Next-Gen Gibson Assembly enables one-step, multi-fragment assembly and straightforward combinatorial libraries with high fidelity.
- Antibody cloning: assemble heavy- and light-chain fragments with a vector backbone in one reaction; move to expression testing the next day
- Library generation: create large pools of enzyme variants or guide-RNA constructs with high accuracy, so diversity screens start with high-quality DNA
- Seamless fusions and tags: scarless joins preserve reading frames for Fc variants, enzyme fusions, and affinity tags
Accelerates biopharma R&D, protein-engineering programs, and labs focused on therapeutic or industrial enzyme evolution.
Automating Molecular Biology
High-throughput labs and synthetic-biology foundries rely on automation to keep pace with build–test–learn cycles. F1-X™ Next-Gen Gibson Assembly is a single-tube, isothermal reaction that drops into robotic liquid-handling and LIMS-linked workflows—robots combine DNA fragments with the F1-X™ Master Mix, incubate, and move on—no thermocycling or multi-step ligations.
- Parallel throughput: assemble dozens of constructs in 96-well plates with consistent, high efficiency—even for many fragments
- Fewer screens: high-fidelity enzymes minimize junction errors, reducing colony screening and sequencing
- Flexible inputs: works with large fragments, low reaction volumes, and difficult sequences across automated protocols
Ideal for synthetic-biology teams, academic core facilities, and biotech startups that want cloning to be a push-button step.
mRNA Development
Rapid mRNA workflows depend on accurate, scarless DNA templates spanning UTRs and antigen or therapeutic sequences. F1-X™ Next-Gen Gibson Assembly links these elements into a single plasmid in one step—with no extra bases at junctions and high-fidelity performance.
- Template accuracy: high-fidelity enzyme system keeps error rates low so IVT mRNA matches the design
- Speed to bench: go from sequence design to in vitro–transcribed mRNA in about a day, accelerating vaccine and therapeutic R&D
- Streamlined complexity: assemble long 5′/3′ UTRs, coding regions, and poly(A) tails in one step; ready for transcription and LNP formulation
Perfect for vaccine developers, therapeutic mRNA teams, and academic groups exploring next-generation RNA medicines.
RC-X™ Rolling Circle Amplification
Cell-free plasmid amplification directly from Gibson assemblies. Generate µg-scale DNA in 2–6 hours—no E. coli recovery or overnight cultures. Ideal for IVT, sequencing, and protein workflows where cells are a drag on productivity.
Phi29-based
E. coli–free
High Yield
V1-X™ High Yield Microbial Protein Expression
A Vibrio natriegens expression system for fast growth and strong titers. T7-system compatible with low endotoxin—built for challenging targets and rapid iteration.
~14-Min DoublingT7-System CompatibleLow EndotoxinReady to upgrade your cloning or amplification workflow?
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