RC-X™ High-Yield Rolling Circle Amplification

RC-X™ High-Yield Rolling Circle Amplification

Microgram-scale DNA. No E. coli. No compromise.

Microgram-scale DNA. No E. coli. No compromise.

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Introduction

RC-X™ is a cell-free DNA amplification kit that delivers >10 µg in 2–6 hours—without minipreps or E. coli. Powered by Phi29 polymerase, it accelerates IVT/mRNA prep, sequencing, and synthetic biology workflows.

Key Features

Fast
2–6 hour turnaround
Clean
Cell-free, low endotoxin
High Yield
Up to 50 µg DNA
Drop-In- Ready
Direct from Gibson Assembly
Automation-Friendly
Ready for liquid handlers

Applications

In vitro transcription & mRNA synthesis

Generate rapid, high-yield DNA templates for IVT. By removing E. coli from the prep, RC-X™ lowers endotoxin risk, improves reproducibility, and compresses timelines from days to hours.

Antibody & vaccine discovery

Support high-throughput screening with isothermal, microgram-scale amplification—no colony picking or minipreps, so design-to-test runs faster.

Sequencing & diagnostics

Produce sequence-ready DNA with minimal background for Sanger or NGS. Low-contamination output aids assay development, variant detection, and construct validation.

Specifications

Coming 2026

Be among the first to use RC-X™, a high-yield, cell-free Rolling Circle Amplification kit for fast DNA prep without E. coli.