F1-X™ Next-Gen Gibson Assembly, 20rxn kit
- $250.00
- $250.00
- (-0%)
- Unit price
- per
Why F1-X™ is Different?
What Makes F1-X™ Different
F1-X™ was developed by Racer with expertise from Dan Gibson, Ph.D., combining the original method with his latest optimizations for fidelity, yield, and multi-fragment performance.
High efficiency on large, multi-fragment builds, including GC-rich and hairpin-containing regions, with scarless junctions.Streamlined setup reduces handling steps and hands-on time—without compromising accuracy.Drops into liquid-handling and LIMS-linked workflows; consistent results across high-throughput runs.Limited Inventor’s Lot with hand-signed packaging from Dr. Gibson, available only in the first production batch.In internal testing, F1-X™ matched or exceeded leading kits on efficiency, fidelity, and time-to-result.
Your success is our priority. With clear protocols and expert guidance, we help you maximize output while minimizing material costs—all with tools designed for reliability and straightforward troubleshooting.Note: F1-X™ Next-Gen Gibson Assembly is in early-access release (Inventor’s Lot). Performance metrics and specifications are being revised during the pre-order validation period; benchmark summaries will be published at launch. Data on file.
How It Works
Step 1: Design Overlaps
Each DNA fragment is engineered with 20–40 bp of overlap at each end, complementary to the adjoining fragment. These overlaps guide correct alignment.
Step 2: Resection
An enzyme chews back the ends of the DNA fragments, revealing single-stranded overhangs.
Step 3: Annealing
The exposed complementary overhangs anneal to each other, aligning the fragments in the desired order.
Step 4: Polymerization
A high-fidelity DNA polymerase fills in any gaps at the junctions, ensuring precise extension across the overlaps.
Step 5: Ligation
A high-fidelity DNA ligase seals the nicks in the backbone, creating a seamless, fully joined double-stranded DNA molecule.
Step 6: Transformation
The completed construct is introduced into E. coli. Any remaining nicks are repaired inside the cell, resulting in a scar-free assembly.
- Choosing a selection results in a full page refresh.